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History of the Department

The Department of Veterinary Microbiology was established since inception of the college in the year 1958 with the aim to impart undergraduate education. Initially only under graduate teaching was under taken. The postgraduate program in Veterinary Microbiology was incepted from 1995 and subsequently in Animal Biotechnology from 2007. The Ph.D program in Veterinary Microbiology was started from 2013. Currently the department offers Undergraduate, Postgraduate and Doctoral degree programs. The curriculum prepares students to meet their goals, which include professional careers in academic, clinical and technical field of Microbiology and Animal Biotechnology. So far 45 students have completed PG programs in Veterinary Microbiology and 17 in Animal Biotechnology. Four students have completed their Ph.D. and another two are undergoing Ph.D. program in Veterinary Microbiology.

The department undertakes time-bound research projects, in addition to the teaching and diagnostic services being provided for diagnosis of bacterial, viral and fungal diseases of livestock and poultry.

Heads of the Department:

Dr. S.L.Manjarekar, Dr. S.P.Phadke, Dr. M. S. Dhake, Dr. M. M. Kulkarni and Dr. R. S. Kolhe initially contributed significantly for the development of the department. Later on Dr. S. D. Harne geared up the developmental activities of the department. Subsequently Dr. D. R. Kalorey played a key role in the overall development and upliftment of the department.Recently Dr.V.C. Ingle is superannuated in March 2020 and Dr.Shubhangi Warke took the charge of Head of the Department.

Sr. No.	Name of The Head	Duration
1.	Dr. S.L.Manjarekar	1958-1964
2.	Dr. S.P.Phadke	1964-1969
3.	Dr. P.R.Dhake	1969-1975
4.	Dr. R. S. Kolhe	1975-1980
5.	Dr. S. D. Harne	1980-1997
6.	Dr. D. R. Kalorey	1997-2014
7.	Dr. V.C.Ingle	2014-2020
8.	Dr.Shubhangi Warke	2020-till date

Present status of the Department

A) Staff position (Teaching and Non –teaching)

Veterinary Microbiology:

Position	Name of faculty	Qualification
Professor	I/c Dr.Shubhangi Warke	M.V.Sc., Ph.D.
Associate Professor	--	--
Assistant Professor	Dr.Shubhangi Warke	M.V.Sc., Ph.D.
Assistant Professor	Dr.P.A. Tembhurne	M.V.Sc., Ph.D.
Sr. Laboratory Technician	Dr. Jiwan Kesarkar	M.V.Sc.

Animal Biotechnology

Position	Name of faculty	Qualification
Assistant Professor	--	--
Senior Research Fellow	Vacant

B) Infrastructure facilities:

i) Laboratories:

Sr. No.	Name of Laboratory	Purpose
1.	Bacteriology and Mycology Laboratory	For carried out separate work of Bacteriology and Mycology.
2.	Veterinary Immunology Laboratory	For carried out separate work of Immunology
3.	Virology Laboratory	For carried out separate work of Virology
4.	Molecular Diagnosis Laboratory	For carried out molecular biology work

ii) Equipments available for Teaching and Research

Sr. No.	Instruments available for Research	Availability
1.	Laminar flow (Vertical /Horizontal)	3
2.	Binocular Microscope	3
3.	Deep fridge ( -20⁰C)	4
4.	Thermal cycler (PCR machine)	2
5.	Gel Documentation system	1
6.	CO₂ Incubator	1
7.	Inverted Microscope	1
8.	Refrigerated centrifuge	2
9.	Deep freezer ( -80⁰C)	1
10.	ELISA reader	1
11.	Spectrophotometer	1
12.	Real Time PCR	1
13.	Gel Electrophoresis apparatus	2
14.	Lyophilizer	1
15.	Hybridization oven	1

Faculty informations

Faculty Information

Dr.Shubhangi Warke (Bhagat)
(MVSc, Ph.D., DBM, NET)
Assistant Professor
Phone – (O) 0712 2510087,
(Fax) 0712 2510883, (M) 9561214395
email : shubhangi_warke@rediffmail.com

Field of specialization : Veterinary Microbiology

Teaching

*UG and PG teaching in Veterinary Microbiology
*PG teaching in Animal Biotechnology
* UG teaching in Fishery College
*Laboratory manuals prepared for Veterinary Microbiology UG & PG Teaching,

*Paper setter for UG theory examinations for different universities

*Participation as External Examiner for different universities.

*Guiding students for ICAR Entrance and interviews

Research:

MVSc research: STUDIES ON EFFECT OF AQUEOUS HERBAL EXTRACTON GROWTH OF ASPERGILLUS OCHRACEOUS, OCHRATOXIN PRODUCTION AND ITS NEUTRALIZATION

Ph.D. research: Genotypic and Phenotypic Characterization of Listeria monocytogenes from bovines and their environment.

P.G. Students Guided:

Major Guide in Veterinary Microbiology: 02

Member of advisory Committee in Veterinary Microbiology

& Animal Biotechnology: 15

Member of advisory committee in other disciplines: 03

Research Projects:.

Completed Research projects:

Title of Project	Funding agency	Amount in Lakhs	Date of Sanction and Duration
Genetic Diversiy of Listeria monocytogenes with different virulence potential ( Principle Investigator)	DST	10.27200/-	2010 -2013
Virulence profiling of Listeria monocytogenes isolated from food-, veterinary and clinical sources (Co- Investigator)	ICMR(Indo German)	10.00/-	2011-2013
ranslational research on Molecular epidemiology of L.monocytogenes (Co- Investigator)	CEIB (DBT)	50.00/-	2012( 5 years)
DBT Network Project on Brucellosis (Co- Investigator)	DBT	1,14,699/-	2012 (5 years )

Completed Sponsored Project

Title of Project	Funding agency	Amount in Lakhs	Date of sfdfdgfdgfdgdfggfggfgf Sanction and Duration
To study the safety & immunogenicity of Brucella abortus RB51, Vaccine in cattle (Co-PI)	IIL	48,750/-	1^st to 31^st November,2010
Evaluation of mastodon in bovine mastitis (CO-PI)	Cattle Remedies India Ltd.	80,040/-	January, 2012 to August ,2012
An open label multicentric clinical trial to study safety and immunogenicity of Tetanus toxoid vaccine in animals	Indian Immunologicals	2.29200/-	March 2019-

PUBLICATIONS :

1) Scientific research paper published in International Journals : 11
2) Scientific research paper published in National Journals : 42
3) Research paper presented at International Seminar :09
4) Research paper presented at National Seminar :86
5) Popular article : 42

ACHIEVEMENTS:

*    Identified herbs with antiochratoxic & OA neutralizing activity
*   Developed Milk based Dot ELISA for detection of   L. monocytogenes
*    Developed Milk based Indirect ELISA for detection of   L. monocytogenes
*    Developed Milk based AB ELISA for detection of   L. monocytogenes
*    Developed Serum based Indirect ELISA for detection of   L. monocytogenes
*   Isolation & characterizations of Listeria from wild life
*   Antibacterial activity of herbal extracts
*   Developed multiplex PCR for detection of virulence marker genes of Listeria spp.
* Identify Disinfectant to reduced contamination of L. monocytogenes at bovine farm.
* Developed Multiplex PCR for detection of antigen of ParvoVirus
* Gene Sequence submitted to gene bank (NCBI) : 55

Recommendation: Research Recommendation -01 in 2019

AWARDS & HONORS:

1.Fellowship :Dr. B.V. Rao WPSA (IB) WPC, 1996. Research Grants 2000 for Post Graduate Research Program.

2. Ayurvet Best Research Article - IX Annual Conference of IAAVR , 2002.

3. Best Poster award – IX Annual Conference of IAAVR, 2002

4. Young Scientist Award -2009 for outstanding service in the field of Veterinary Science

5. World Environment Educational award -2012

6. Mother Teresa Sadbhawana Award-2015

7.Certificae of Appreciation -2016 International Womans Day

8.Appreciation Certificate for participated to organized XXX^th annual Convention& National Symposium of IAVMI at Nagpur from 10-12 th February ,2017.

9.Letter of Appreciation in recognition as a Member of Convocation Cell of eighth convocation of Maharashtra Animal & Fishery Sciences University held during 9 th March ,2017

10 Best poster award- First best Poster Presentation Award during XV Biennial Conference and National Symposium of IAWV at Hyderabad during 21-22 ,November, 2017.

11.First Prize for oral Presentation at National Symposium at Akola during 26^th -27^th November,2019.

12 Second Prize for poster presentation at National Symposium at Akola during 26^th -27^th November,2019.

13 Letter of Appreciation: for worked as Member in judging Committee for Elocution & Drawing Competition held on the occasion of “World Animal Day” on 4^th October, 2019, at Nagpur Veterinary College, Nagpur .

14. Appreciation Certificate for outstanding contribution and dedicated efforts for successful organization of National conference & XVI Annual Symposium of IAVPHS on 26 and 27 th February,2019 at NVc., Nagpur

15.Certificate of Honour for Best Paper Presentation award in Canine Microbiology & Immunology Session 2019 In XVII National Congress on Canine Practice & Natiional Symposium at Jabalpur(M.P.) during 21-23 Jan 2020.

Organization of Event:

1.Organized 30^thAnnual Convention of Indian Association of Veterinary Microbiologists Immunologists and Specialist in Infectious Diseases (IAVMI) and National Symposium on “Challenges in Animal Health for Higher productivity and Income to farmers” during 10-12^th Feb 2017.------- as Joint secretary

2. Associated with Training and Workshop organized by Department:08

EXTENSION:

Extention and Field Outreach Activities : Technical guidance to farmers and Field Veterinarians through camps, lectures, trainings, radio talk , T.V. talks & visit at adopted villages etc.

Dissemination of technical knowledge through article publications in local news paper , leaflets & pamphlets

MEMBERSHIP:

Membership of Scientific organizations (8),

1) Life member of Indian association of Veterinary Microbiologist,Immunologist and specialist in Infectious Diseases.(IAVMI)
2) Life Member of Indian association for the Advancement of Veterinary Research (IAAVR)
3) Life member of Indian association of Veterinary Public Health Specialist (IAVPH),
4) Society for Immunology &Serology
5) Indian Veterinary Community
6)   Life member of National association for welfare of Animal Research
7) Life Member of Society for Listeria
8)   Life Member of Indian Association for Woman’s Veterinarians’ (IAWV
9)  Life Member of Indian Society of Canine Practitioner

Dr. Prabhakar A Tembhurne,
(MVSc, PhD, PDF(USA))<
Assistant Professor
Phone – (O) 0712 2510087, (Fax) 0712 2510883, (M) +91 9765712029
email:prabhakar.tembhurne@gmail.com

Field of specialization: Veterinary Virology

Field of Interest

small RNA biology (miRNA), Molecular diagnostics & epidemiology of animal pathogens, Host-pathogen interaction.

Teaching

UG ,PG and PhD teaching in Veterinary Microbiology using audiovisual and Web based tools.

PG teaching in Animal Biotechnology using audiovisual and Web based tools

Manuals prepared for Animal Biotechnology and Veterinary Microbiology courses.

Research

M.V.Sc. (IVRI) Research – Studies on the development of inactivated monovalent vaccine against Bluetongue virus.

Ph.D. (IVRI) Research – Molecular characterization of L2 and M5 genes of Bluetongue virus serotypes 18 and 23.

Post Doctoral Research 2004-2007 (North Carolina State university, Raleigh ,USA):

Functional Genomics: miRNA profiling in developmental chicken embryo: Deciphering role of antimeq gene in MDV pathogenesis

Research Scientist: (National AIDS Research Institute, Pune):

HPTN/ACTG clinical monitoring of Viral Load in HIV patients

Post-graduate Student Guided:
Major advisor: PG (M.V.Sc) In Veterinary Microbiology) : 03
PG (M.V.Sc ) In Animal Biotechnology) : 06
MSc : 02
PhD In Veterinary Microbiology: 01
Member of Advisory: PG (M.V.Sc ) : 35 PhD : 5

Research Project:

Title of Project	Funding Agency	Amount	Date of sanction and Duration
Completed Projects:
Principle Investigator Understanding the molecular basis of peste-des-petits ruminants virus (PPRV) mediated host immune modulation of the development of next generation vaccines	NASF- ICAR, Govt. of India, New Delhi	49.12 lakhs	2017-20
Co- Investigator: (Lead Centre PI & Coordinator in 2020) “Molecular Surveillance and Cross Species Transmission of Rotaviruses from Animal and Human Origin from Maharashtra	DBT, Govt. of India,New Delhi	50 lakhs	2017-20
Co-Investigator: “Centre for Zoonosis	ICAR-ICMR,Govt. of India, New Delhi	437.608 lakhs	2015-2017
Co- Investigator: “Molecular epidemiology of Brucellosis”	DBT, Govt. of India, New Delhi	114 lakhs	2012-15

Principle Investigator Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.	DST, Govt. of India, New Delhi	20 Lakhs	2012-15
Co- Investigator: Epidemiological studies on classical swine fever in central India & Goa.	DBT, Govt. of India, New Delhi	105 Lakhs	2009-2013

Research Achievements:

1) Research Recommendations : 03

2) Published Full Genome of IBDV circulating in Nagpur Region

3) Developed NEWS paper/ordinary Filter based DSS transport & detection methodology

for the diagnosis of Peste-des-petits-ruminants virus.

4) Identified novel miRNAs in PPRV infections and miRNAs as prognostic biomarker for

PPRV infections.

5) Deciphered microRNAs (miRNA) role in embryonic immune organ developmental

pathology by Knockdown miRNA & in immune competency in immune suppressed

chicks by therapeutic miRNA.

6) Identified novel microRNAs in developing chicken embryos and in immunological important organs in chicken.

7) Contributed for development of Recombinant protein based ELISA (Launched by ICAR) for diagnosis of Bluetongue virus (BTV).

8) First to define the emergence of polyclonal escape mutant of BTV leading to thrust for adaptation of molecular tools for characterization of BTV over serological typing.

9) First to published full length genetic makeup important viral genes of BTV serotypes 18 & 23

10) First to develop a successful BEI inactivated experimental vaccine for BTV Indian isolates.

Awards & honors:

Recipient of BHARAT RATNA SIKSHA AWARD conferred by Global Society for Heath & Educational Growth, New Delhi.

Best Poster Award :-IAVMI 2019.

Publications:

Research Publications: International (Peer reviewed Journals): 7, National 28

Other Publications/Presentations in Conferences/Seminars –

International: 9 National: 40

Popular articles /manuals etc: 5

Gene sequences Submitted: 54

Member of Scientific Society:

Indian Association for Advancement of Veterinary Research

Indian Association for Veterinary Immunolgy and Microbiology

Extension:

Extension and Field Outreach Activities: technical guidance to farmers, field veterinarian through camps, lectures, trainings & visits at adopted villages etc.

Dissemination of technical knowledge through extension publications in local language, manuals etc.

Diagnostic Service : For animal diseases diagnosis for viral,bacterial and fungal disease

Currently in COVID-19 diagnosis at ICMR approved NVC,Nagpur Lab.

Extension project : 01

Title of Project

Funding Agency

Amount ( lakhs)

Date of sanction and Duration

Completed Project:

Co-Investigator:

Establishment of community based Animal Husbandry Practices in Tribal Village of Gadchiroli District of Maharashtra State

ICAR, New Delhi

36.33 lakhs

2014-15

Achievements of the Department

Achievements of the Department:

Infrastructure development for Education and Research in Veterinary Microbiology & Animal Biotechnology (UG,PG & PhD)
Development of HRD in Animal Biotechnology through national programme.
Establishment of Laboratory Animal Experimental Facility.
Repository of bacterial & viral isolates viz. Staphylococcus aureus, Listeria monocytogenes, E. coli and Parvovirus, Poxviruses, IBD etc
Development of in-house assays for diagnosis of infectious diseases viz. Sample collections (Dried spot sample PCR using newspaper /filterpapers) –DSS PCR); Multigene based identification of PPRV for better confirmation of disease in Indian scenario).
Antigen detection of Parvovirus by Multiplex PCR
Molecular epidemiology of Staphylococcus spp., Listeria, spp. Rotaviruses and Poxviruses, PPR, and IBD from Maharashtra State.
Identified herbs for neutralization and inhibition of aflatoxin and ocrotoxin A.
Deciphered role of miRNA in therapy of immunocompromized chicken.
Development of Milk (Dot, Indirect & AB) and Serum(Indirect) ELISA for detection of L. monocytogenes

Isolation & characterizations of Listeria from wild life
Identify Disinfectant to reduced contamination of L. monocytogenes at bovine farm.
Several Gene Sequence submitted to gene bank

Facility created:

High end research facilities viz. equipments ( Real time PCR, Thermal Cyclers, Hybridization oven, Electrophoresis systems, Biosafety cabinets, CO₂ incubators, etc)
Establishment of Laboratory Animal Experimental Facility.
Cell culture facility for virology works
Molecular biology facility ( DNA/RNA/Protein works)

Scientific Collaborations

Central India Institute for Medical Sciences, Nagpur (MOU signed)
Apex research institutes in Veterinary Sciences, like IVRI, Izatanagar, NIVEDI, Bangalore, National Institute of Virology, Pune etc
Indira Gandhi Government Medical College, Nagpur
Government Medical College, Nagpur
Mahatma Gandhi Institute of Medical Sciences, Sewagarm, Wardha
Veterinary Colleges of different states
IISER, Mohali
Delhi University (South Campus)
Indian Immunologicals

Vision / Mission of the Department

Course offered / Conducted by the department

Course Offered / Conducted by the Department:

U.G. course : Veterinary Microbiology ( MSVE -2016) 3+2=5 II nd Year of BVSc.& AH

P.G.Courses: (In Vety.Microbiology)

Sr. No.	Corse No.	Title	Credits	Semester
1	VMC-601	Bacteriology -I	3+1=4	I
2	VMC-602	Bacteriology -II	3+1=4	II
3	VMC-603	Veterinary Mycology	1+1-2	II
4	VMC-604	General Virology	2+1=3	I
5	VMC-605	Systematic Animal Virology	3+1=4	II
6	VMC-606	Principles of Immunology	2+1=3	I
7	VMC-607	Vaccinology	2+0=2	III
8	VMC-608	Diagnostics of Infectious Diseases	1+2=3	III
9	VMC-609	Techniques in Microbiology & Immunology	0=3=3	I
10	VMC-691	Masters Seminar	1+0=1	III
11	VMC-699	Masters Research	20	III & IV

P.G. Courses : (In Animal Biotechnology)

Sr. No.	Corse No.	Title	Credits	Semester
1	ABT-601	Basic & Applied Biotechnology	3+0=3	I
2	ABT-602**	Fundamentals of Cell & Molecular Biology	3+0=3	I
3	ABT-603	Applied Molecular Biology	2+1-3	II
4	ABT-604**	Animal Cell Culture; Principles & Applications	1+2=3	I
5	ABT-605**	Molecular Diagnostics	1+2=3	II
6	ABT-606	Vaccine Biotechnology	2+0=2	III
7	ABT-607	Immunology Applied to Biotechnology	1+1=2	III
8	ABT-608	Introduction to Bioinformatics	1+1=2	III
9	ABT-609**	Animal Genomics	2+1=3	II
10	ABT-610**	Reproductive Biotechnology	2+1=3	III
11	ABT-611**	Techniques in Molecular Biology and Genetic Engineering	0+3=3	II
12	ABT-613	Molecular Forensics	2+1=3	III
13	ABT-616	Animal Biotechnology	0+3=3	II
14	ABT-691	Masters Seminar	1+0=1	III
15	ABT-699	Masters Research	20	III & IV

** Compulsory courses

Ph.D.Courses: (In Vety.Microbiology)

Sr. No.	Corse No.	Title	Credits	Semester
1	VMC-701	Advances in Bacteriology	2+1=3	I
2	VMC-702	Advances in Mycology	2+1=3	I
3	VMC-703	Bacterial Genetics	2+1=3	I
4	VMC-704	Microbial Toxins	2+1=3	II
5	VMC-705	Molecular Determinants of Bacterial Pathogenesis	2+1=3	II
6	VMC-706	Advances in Virology	2+1=3	I
7	VMC-707	Molecular and Genetic Aspects of Viral Pathogenesis	2+1=3	II
8	VMC-708	Structure Function Relationship of DNA and RNA Viruses	3+0=3	II
9	VMC-709	Oncogenic Viruses	2+0=2	III
10	VMC-710	Slow Viral Infections and Prions	2+0=2	III
11	VMC-711	Molecular Immunology	2+1=3	I
12	VMC-712	Advances in Cellular Immunology	2+1=3	I
13	VMC-713	Cytokines and Immunomodulators	2+0=2	II
14	VMC-714	Advances in Vaccinology	2+0=2	III
15	VMC-715	Advances in Immunodiagnostics	1+1=2	III
16	VMC-716	Modern Immunotechnology	1+2=3	III
17	VMC-717	Current Topics in Infection and Immunity	3+0=3	III
18	VMC-718	Veterinary Microbial Biotechnology	2+1=3	II
19	VMC-790	Special Problem	0+2=2	IV
20	VMC-791	Doctoral Seminar I	1+0=1	II
21	VMC-792	Doctoral Seminar II	1+0=1	III
22	VMC-799	Doctoral Research	45

Departmental Research Activity

Departmental Research Activity :

Research programmes:

i) Completed Research Projects

Sr. No.	Type	Principle Investigator	Title	Period	Cost (Rs in Lakhs )
1.	NATP(MM)	Dr. D.R.Kalorey Dr.V.C.Ingle	Animal Health information system through disease monitoring and surveillance	2000-04	19.80
2.	NATP(CGP)	Dr. D.R.Kalorey Dr.V.C.Ingle	Development of ELISA for detection of L. monocytogenes from milk.	2002-05	14.09
3.	DBT Network	Dr. D.R.Kalorey	Development of Standardized herbal product for bovine mastitis	2006 -11	43.22
4.	DST(SERB- Young Scientist)	Dr.S.R. Warke	Genetic diversity of Listeria monocytogenes with different virulence potential	2010-13	10.00
5.	Indo German ICMR	Dr.D.R.Kalorey Dr.N.V.Kurkure Dr.S.R. Warke	Virulence profiling of Listeria monocytogenes isolated from food, veterinary and clinical sources.	2011-13	10.66
6.	DBT	Dr.D.R.Kalorey Dr. N.V. Kurkure Dr. P.A. Tembhurne Dr.ShubhangiWarke Dr. S.P.Awandkar	DBT Network Project on Brucellosis.	2012-16	114.70
7.	DST	Dr. P.A. Tembhurne	Deciphering role of miRNA in chicken developmental pathology & its evaluation as therapeutic in immune suppressed chicks.	2012-15	18.00
8.	DBT-HRD	Dr.V.C.Ingle	Development HRD in Animal Biotechnology.	2010-onwards	109.02
9.	DBT	Dr.D.R.Kalorey Dr.N.V.Kurkure Dr. S. R. Warke	Translational research on Molecular epidemiology of L. monocytogenes.	2012-2017	57.70
10.	ICAR-TSP	P. A. Tembhurne ( Co-PI)	Establishment of community based animal husbandry practice in tribal villages of Gadchiroli district of Maharashtra State	2014-2017	206.00
11	ICAR-ICMR	Dr.V.C.Ingle ( Co-PI) P. A. Tembhurne ( Co-PI) Dr.S.P.Awandkar ( Co-PI)	Centre for Zoonoses (Niche Area of Excellence)	2015-2019	407.00
12	ICAR NASF	Dr. P.A.Tembhurne (Co-operating centre PI)	Understanding the molecular basis of peste-des-petits ruminants virus (PPRV) mediated host immune modulation for the development of next generation vaccines	2016-2019	300.00
13	ICMR	Dr. V.C. Ingle	Molecular epidemiology and cross species transmission of rotavirus of animal and Human origin from Maharashtra	2017-2020	70.00

ii)Ongoing Research Projects:

Sr.

No.

Type

Principle Investigator

Title

Period

Cost (Rs in lakhs)

DBT

Department of Veterinary Microbiology

Star College Scheme of DBT

2020-2022

19.00

ii) Research Projects submitted to various funding agencies

Sr. No.	Title of Project	Investigators	Duration/ Date of submission	Funding Agency	Total Outlay (in lakhs)
1.	Molecular epidemiology & development of multivalent vaccines against Bovine Mastitis caused by Staphylococcus aureus	Dr.D.R.Kalorey	3 yrs	MBC	221.70
2.	Development of Multiple Dot –ELISA for simultaneous diagnosis of reproductive diseases.	Dr.D.R.Kalorey	3 yrs	MBC	102.21
3.	Molecular characterization of Avian pathogenic Escherichia coli	Dr.D.R.Kalorey	3 yrs	MBC	192.10
4.	Molecular characterization of Brucella strains from reproductive disorders of cattle, buffalo and goat	Dr. V.C.Ingle	3 yrs	MBC	90.56
5.	Development of standardized vaccine for Staphylococcal bovine mastitis in India.	Dr.D.R.Kalorey	3 yrs	BIPP	12.119744 Crores (2.747624 crores)
6.	Development of vaccine against bovine mastitis caused by CNS	Dr.D.R.Kalorey	3 yrs	RGS& T C	67.14
7.	Development of multiplex PCR for detection of enterotoxigenic Staphylococcus aureus from milk and milk products	Dr.D.R.Kalorey	3 yrs	RGS& T C	59.14
8.	Molecular Epidemiology and development of vaccine against bovine mastitis	Dr.D.R.Kalorey	3 yrs	DBT Network	612.24
9.	Central Disease Diagnostic Laboratory	Dr.D.R.Kalorey	3 yrs	ICAR	56.00
10.	Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.	Dr. P.A.Tembhurne	3 yrs	DST-FAST TRACK	24.672
11.	Evaluation of antiviral activity of Growell liquid against Fowl pox virus.	Dr. P.A.Tembhurne	6 months	Growell India	1.20
12.	Centre for Animal Disease Diagnosis, Research and Control	Dr.D.R.Kalorey	5 Years	RKVY	461.50
13.	Safety and immunogenicity of Brucella abortus RB51 vaccine in cattle and buffaloes	Dr.D.R.Kalorey	3 Years	DBT	55. 648
14.	Development of serological and DNA based diagnostic tools for brucellosis	Dr.D.R.Kalorey	DBT	DBT	71.686
15.	*miRNA profiling of canine transmissible venereal tumor : A novel markers in body fluids for early diagnosis of cancer	Dr. P. A. Tembhurne Dr.V.C.Ingle Dr. S. P. Awandkar Dr.G.R.Bhojne	3Yrs	DBT	32.00
16	Molecular markers to identify pathogenic Listeria monocytogenes from animal food origin & human clinical cases.	Dr. S.R. Warke Dr.V.C. Ingle Dr. N.V. Kurkure	3 Yrs 11-4-2014	DBT	48.84
17	Development of Infrastructure Facility for Animal Disease Diagnosis and Molecular Characterization of Pathogens	Dr.V.C.Ingle Dr. P. A. Tembhurne Dr. S. P. Awandkar Dr.U.M.Tumlam Dr.S.R.Warke Dr.V.K.Basunathe Dr.G.R.Bhojne Dr.P.M.Sonkusale	3Yrs 13-10-2015	RKVY	282.8
18.	*Decipering molecular interaction of Infectious Bursal Disease Virus (IBDV) with cellular genes and host encoded miRNA	Dr. P. A. Tembhurne	21-10-2015	ICAR
19.	Studies on Molecular Epidemiology of Pestiviruses in small ruminant	Dr.V.C.Ingle	2 yrs 11-9-2015	ICAR Ad-hoc	40 .00
20.	Epidemiology and Impact Assessment Of Infectious Bovine Rhinotrachitis in Livestock of Central India	Dr.S.P.Awandkar	2 yrs 11-9-2015	ICAR Ad-hoc	40 .00
21.	Studies on cross species transmission of Rotaviruses in livestock	Dr.V.C.Ingle	6 m 8-2-2016	NAAS short term study	1.00
22	Disinfectant Resistant of L.monocytogens from bovine environment	Dr.S.R.Warke	6 m 8-2-2016	NAAS short term study	1.00
23.	Status of clean milk production at organized and non-organized farms in Bhandara district	Dr.S.P.Awandkar	6 m 8-2-2016	NAAS short term study	1.00
24.	Curing of plasmid from CPS & CNS isolated from Bovine Subclinical Mastitis using Kamdhenu ark	Dr.U.M.Tumlam	6 m 8-2-2016	NAAS short term study	1.00
25.	Evaluation of cheapest cellular matrix base materials viz. News paper/filter paper for room temperature PPR sample dispatch and its molecular characterization	Dr.P.A.Tembhurne	6 m 8-2-2016	NAAS short term study	1.00
26.	Holistic approach to identify common infectious agents of diarrhea in dogs	Dr.P.A.Tembhurne	21-6-2016	DBT
27.	Studies on Molecular epidemiology of IBR virus in cross bred bovines of Maharashtra state	Dr.V.C.Ingle	3 Years 11-7-2016	RGSTC	20.00

Agency Projects undertaken by the Department

Sr. No.	Agency	Principle Investigator	Title	Period	Cost (Rs)
1.	Growell India Pvt.Ltd. Pune	Dr.D.R.Kalorey	Role of Growell as immuno modulator in IBD vaccinated Chicks.	1997	18,950/-
2.	Growell India Pvt.Ltd. Pune	Dr.D.R.Kalorey	Immunomodulatory effect of Growell during experimental Aflatoxicosis in chicks.	1997	26,880/-
3.	DaburAyurvet Ltd. Gaziabad -10	Dr.D.R.Kalorey	Effect of herbomineral premix on fungal Growth & in vitro inhibition of aflatoxin B1 production.	1998	30,500-/
4.	DaburAyurvet Ltd. Gaziabad –10	Dr.D.R.Kalorey	Effect of herbomineral toxin binder during experimental mycotoxicosis in broiler chicks.	2000	30,500/-
5.	Kemin Nutriti onal Technolo gies,(India)	Dr.D.R.Kalorey	Efficacy of Toxin-binder against aflatoxicosis in broilers.	2002	30,000/-
6.	Dabur Ayurvet Ltd Gaziabad – 10	Dr.D.R.Kalorey	In vivo efficacy of herbal preparation during simultaneous mycotoxicosis in Broilers.	2002	15,000/-
7.	Dabur Ayurvet Ltd Gaziabad – 10	Dr.D.R.Kalorey	.Evaluation of efficacy of mastilep during subclinical mastitis	2002	10,000/-
8.	M/s Intervet Pvt. Ltd.,Pune	Dr.D.R.Kalorey	Evaluation of Brucella RB 51 vaccine under field condition.	2005	60,000/-
9.	M/s Intervet Pvt. Ltd.,Pune	Dr.D.R.Kalorey	Evaluation of safety and efficacy of oil and gel adjuvanted BOVILS (R) HSBQ vaccine in cattle under field condition.	2007	25,000/-
10.	Bentoli Agri Nutrition Asia Pvt. Ltd	Dr.D.R.Kalorey	Evaluation of antibacterial and antifungal activity of antimicrobial product.	2007	1,65,865/-
11.	Bentoli Agri Nutrition Asia Pvt. Ltd	Dr.D.R.Kalorey	Antifungal and antimycotoxic activity of mold inhibitor	2007	38,080/-
12.	Indian Immunologicals Limited	Dr.D.R.Kalorey	To study Safety and immunogenicity of Brucella abortus RB51 in cattle	2009	48,750/-
13.	Cattle Remedies, India Ltd.	Dr.D.R.Kalorey	Evaluation of Mastodin in bovine mastitis	2011	80,000/-
14	IIL, Hyderabad	Dr.S.R.Warke	An open label multicentric clinical trial to study safety and immunogenicity of Tetanus toxoid vaccine in animals	2019-2020	2.29

Recommendations:

Sr. No.	Project No.	Research Workers	Recommendations
1	NVC/Micro/02/2004	Dr. D.R.Kalorey Dr.Jyoti Bodade	Chicks vaccinated at 7^th day of age with inactivated emulsified ND+IBD vaccine containing 10% & 20% Vit E are protected against New Castle Disease.
2	NVC/Micro/01/2007	Dr. D.R.Kalorey Dr.P.K.Jallewar	Norfloxacillin & Chloramphenicol can be used in Listeria infection, since the prevalence of Listeria species in fresh water fish Vagur (Clavrus batracus) was 19.50% and of Listeria monocytogenous 13.00 per cent.
3	NVC/Micro/02/2007	Dr. D.R.Kalorey Dr. Deepti K.Pillai	Antigen capture Sandwich ELISA developed for the detection of Pasteurella multocida antigen is sensitive (81.25%) specific ( 100%) and easy to perform, hence can be used as an efficient diagnostic tool for the diagnosis of fowl cholera from serum.
4	NVC/Micro/02/2015-16	Dr. P.A Tembhurne Dr. M.A.Pundlik	Ink free news paper or filter paper of around 1.5cm diameter spotted with nasal secretion of affected animals was found suitable for diagnosis of Peste des Petits Ruminants Virus by RT-PCR.
5	NVC/Micro/02/2016-17	Dr. P.A Tembhurne Dr. R. Narayan	For Detection of PPRV (Peste des Petits Ruminants Virus) in field samples, RT-PCR technique targeting both the M & N gene should be employed.
6	NVC/Micro/ /2018-19	Dr.Shubhangi Warke	The disinfectant Didocyldiamethyl ammonium chloride (DAC), found to be most effective against the L. monocytogenes, lower that the recommended concentration
7	NVC/Micro/ /2018-19	Dr. P.A Tembhurne Dr. Pooja Acharya	The multiplex PCR approach for amplification of cytb of tiger & leopards and its sequencing from scat sample easily obtained sample in wild as well as captivity will be a tool useful for identification of endangered species

Publications:

1) Scientific research paper published International : 11, National : 127

2)Research paper presented at seminar International : 15, National :117

3)Popular article : 42

4) Prepared Manual on Milk Dot ELISA for detection of L. monocytogenes.

5) Prepared Manual on Milk Indirect ELISA for detection of L. monocytogenes.

6) Prepared Manual on Milk Avidin Biotin ELISA for detection of L. monocytogenes.

7) Prepared Manual on Serum ELISA for detection of L. monocytogenes.

8) Prepared SOPs of Diagnostic protocol

9) Prepared Monograph: 05

10) Prepared Leaflets:10

B) Area of Research

Seminar/Conference / Organized:

Sr. No.	Seminar/Conference /Training organized	Date
1.	IX Annual Conference of Indian Association for the Advancement of Veterinary Research (IAAVR) and National Symposium on “Concepts in Sustainable Livestock Production and Health in New Millennium”	4-5^th Feb 2002
2.	National symposium on “Zoonoses and Biotechnological Applications”	4-5^th Feb.2008
3.	30^thAnnual Convention of Indian Association of Veterinary Microbiologists Immunologists and Specialist in Infectious Diseases (IAVMI) and National Symposium on “Challenges in Animal Health for Higher Productivity and Income to Farmers”	10-12^th Feb 2017

Training/ Workshop/Popular Lecture

Sr. No.	Seminar/Conference /Training organized	Date
1.	Popular Lecture Series on Biotechnology.	4^th Oct.2007
2.	Work shop on “Dairy Management & Nutrition”	24^th Aug. 2007
3.	Short term training course on “Application of Molecular Methods in Livestock Disease Diagnosis and Research”	17^th-31^st July,2008
4.	Short term Training course on “Diagnosis of Brucellosis” under the Continuing Veterinary Education Programme funded by Veterinary Council of India.	25-28^th May 2009
5.	Short term Training course on “Diagnosis of Brucellosis” under the Continuing Veterinary Education Programme funded by Veterinary Council of India, New Delhi.	1-4^th June 2009
6.	Three day workshop on “Care, Breeding and Management of Laboratory Animals.	15-17^th Jan 2009
7.	Work shop on Bioinformatics and PCR.	19-20^thMarch 2010
8.	One day workshop on ”Dugdhotpadan: Krishi Jivnacha Shashwat Aadhar.	26^th Dec 2014
9.	Entrepreneurial Seminar on “Large scale production of anti snake venom”	6^th June 2015

Diagnostic services catered

Diagnostic services are catered on payment basis with the following charges.

Sr.No.	Diagnostic Tests	Amount (Rs)
1.	Cultural Examination & AST	200.00
2.	Biochemical characterization of bacteria	200.00
3.	Smear examination	100.00
4.	Skin/hair for mycotic infection	300.00
5.	Mycotoxin in feed	500.00
6.	Brucellosis by RBPT/Salmonellosis by PAT	50.00 / Sample
7.	Brucellosis by STAT	100.00/ Sample
8.	Brucellosis by ELISA	500.00/ Sample
9.	Tuberculin/Johnin test	200.00 / animal
10.	Seromonitoring (RD)	500.00 / 5 sera
11.	MPN	200.00
12.	Plate count	300.00
13.	CMT reagent	250.00/ 500 ml
14.	Subclinical mastitis	20.00
15.	Attending diseases outbreak & investigation	2000.00
16.	Consultancy during outbreak / Visit	500.00

Departmental Extension Activity

Lab Photo

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